Study of some Biochemical Parameters and Partial Purification of amylase from diabetic patients

The aim of the study was to evaluate level amylase in serum diabetic patients compared healthy controls, glucose, lipids, lipoproteins, thyroid hormones, electrolytes (calcium, potassium, and sodium) transporter enzymes were also studied. (AST), (ALT),(ALP). enzyme purified association between previously mentioned variants it studied other biochemical variables found that there a significant positive correlation when comparing with (TG, VLDL, ALP) no correlation. activity variables. Where one peak obtained fourth part purification, degree purification 16.1; result 108.2% specific (0.189 ng/ mg, kinetic studies partially enzyme. substrat concentration 10 ng/mg, value Michaelis Menten’s constant km (5.55 ng) maximum velocity Vmax (0.98 ng/ml) optimum temperature reached (37 °C) while pH (7.5).